Advantages of Tissue Slices
A precision-cut tissue slice represents an in vitro model which mimics closely the multi-cellular complexity, extra-cellular interactions, structural and functional features of the whole organ. The architectural composition of the various cell types is retained as well as the cell-matrix and cell-cell interactions. These slices retain the biochemical capacity and the functional heterogeneity allowing for the assessment of total metabolism and potential toxicity of a compound under conditions most similar to the in vivo situation.
- Higher level of biological organization which may better reflect the response of the target organ
- A differentiated state which is based on cell-cell and cell-matrix interaction
- Maintenance of functional heterogeneity and biochemical capacity
- Preservation of Phase I and Phase II drug metabolism reactions
- Applicability of the tissue slice system to donated, non-transplantable human tissue
Human and Animal Tissue Slice Uses
The use of human tissue slices in drug biotransformation, organ specific toxicity, and environmental contaminant studies is more relevant for predicting what may be seen in humans. The multi-cellular and extracellular matrix composition of tissue slices reflect the biologically relevant structural and functional features of in vivo tissue to facilitate and advance mechanistic investigations. Drug induced time and concentration dependent changes in tissue slice function, gene expression, and morphology that lead to organ injury and repair have the potential to more accurately reflect, as well as predict, in vivo outcomes. The data obtained can help in drug candidate selection, identifying sensitive biomarkers of side effects and augment the extrapolation and translation of animal and human in vitro results into clinical applications and predictions. Gaining knowledge about human response and the range of responses (normal, stressed, diseased) will aid in defining human variability and susceptibility to drug induced organ injury and environmental toxins in comparison with animal models.
- Drug induced organ specific toxicity
- Mechanisms of drug induced organ injury
- Drug uptake and biotransformation
- Pathways of cellular repair and regeneration
- Characterization of species drug and environmental contaminant response and susceptibility
- Co-culture of different organ tissue slices
- Cold- and cryo- preservation methods